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KMID : 0379119980260020239
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Korean Journal of Mycology
1998 Volume.26 No. 2 p.239 ~ p.245
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Effects of Mixed Carbon Sources on the Production of Cellulase by Trichoderma reesei
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Abstract
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The feasibility of enzymatic hydrolysis of cellulosic materials is dependent on the cost of cellulase, which is strongly influenced by the selection of proper carbon source in the cellulase production medium. When solka floc was used as a carbon source for the production of cellulase by Trichoderma reesei Rut C-30, a maximum of 53.2 U/㎖ of CMCase activity (4.8 U/㎖ of FPase activity) was obtained with a concentration of 1 % of solka floc. The cellulase activity decreased to 50% in the presence of 0.5% of glucose in the medium. The production of cellulase was considerably enhanced when solka floc and wheat bran were used together as a carbon source. A medium which contained 1% of solka floc and 3% of wheat bran yielded highest cellulase activity: CMCase activity of 76 U/㎖ and FPase activity of 12.5 U/㎖.
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